Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
Identifieur interne : 000084 ( Main/Exploration ); précédent : 000083; suivant : 000085Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
Auteurs : Genevieve Housman [États-Unis] ; Joanna Malukiewicz [États-Unis, Brésil] ; Vanner Boere [Brésil] ; Adriana D. Grativol [Brésil] ; Luiz Cezar M. Pereira [Brésil] ; Ita De Oliveira E Silva [Brésil] ; Carlos R. Ruiz-Miranda [Brésil] ; Richard Truman [États-Unis] ; Anne C. Stone [États-Unis]Source :
- PLoS Neglected Tropical Diseases [ 1935-2727 ] ; 2015.
Abstract
Zoonotic pathogens that cause leprosy (
Url:
DOI: 10.1371/journal.pntd.0004198
PubMed: 26571269
PubMed Central: 4646627
Affiliations:
- Brésil, États-Unis
- Arizona, Louisiane, Minas Gerais, Pernambuco, État de Rio de Janeiro
- Rio de Janeiro
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Grativol</name><affiliation wicri:level="3"><nlm:aff id="aff003"><addr-line>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro</wicri:regionArea><placeName><settlement type="city">Rio de Janeiro</settlement><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author><author><name sortKey="Pereira, Luiz Cezar M" sort="Pereira, Luiz Cezar M" uniqKey="Pereira L" first="Luiz Cezar M." last="Pereira">Luiz Cezar M. Pereira</name><affiliation wicri:level="2"><nlm:aff id="aff004"><addr-line>Centro de Conservação e Manejo de Fauna da Caatinga, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Centro de Conservação e Manejo de Fauna da Caatinga, Universidade Federal do Vale do São Francisco, Petrolina, Pernambuco</wicri:regionArea><placeName><region type="state">Pernambuco</region></placeName></affiliation></author><author><name sortKey="Silva, Ita De Oliveira E" sort="Silva, Ita De Oliveira E" uniqKey="Silva I" first="Ita De Oliveira E" last="Silva">Ita De Oliveira E Silva</name><affiliation wicri:level="2"><nlm:aff id="aff005"><addr-line>Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, Minas Gerais</wicri:regionArea><placeName><region type="state">Minas Gerais</region></placeName></affiliation></author><author><name sortKey="Ruiz Miranda, Carlos R" sort="Ruiz Miranda, Carlos R" uniqKey="Ruiz Miranda C" first="Carlos R." last="Ruiz-Miranda">Carlos R. Ruiz-Miranda</name><affiliation wicri:level="3"><nlm:aff id="aff003"><addr-line>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil</addr-line></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratório de Ciências Ambientias, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro</wicri:regionArea><placeName><settlement type="city">Rio de Janeiro</settlement><region type="state">État de Rio de Janeiro</region></placeName></affiliation></author><author><name sortKey="Truman, Richard" sort="Truman, Richard" uniqKey="Truman R" first="Richard" last="Truman">Richard Truman</name><affiliation wicri:level="2"><nlm:aff id="aff006"><addr-line>HHS\HRSA\HSB National Hansen's Disease Program-NIAID IAA-2646, Baton Rouge, Louisiana, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>HHS\HRSA\HSB National Hansen's Disease Program-NIAID IAA-2646, Baton Rouge, Louisiana</wicri:regionArea><placeName><region type="state">Louisiane</region></placeName></affiliation></author><author><name sortKey="Stone, Anne C" sort="Stone, Anne C" uniqKey="Stone A" first="Anne C." last="Stone">Anne C. Stone</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona</wicri:regionArea><placeName><region type="state">Arizona</region></placeName></affiliation></author></analytic><series><title level="j">PLoS Neglected Tropical Diseases</title><idno type="ISSN">1935-2727</idno><idno type="eISSN">1935-2735</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Zoonotic pathogens that cause leprosy (<italic>Mycobacterium leprae</italic>) and tuberculosis (<italic>Mycobacterium tuberculosis</italic> complex, MTBC) continue to impact modern human populations. Therefore, methods able to survey mycobacterial infection in potential animal hosts are necessary for proper evaluation of human exposure threats. Here we tested for mycobacterial-specific single- and multi-copy loci using qPCR. In a trial study in which armadillos were artificially infected with <italic>M</italic>. <italic>leprae</italic>, these techniques were specific and sensitive to pathogen detection, while more traditional ELISAs were only specific. These assays were then employed in a case study to detect <italic>M</italic>. <italic>leprae</italic> as well as MTBC in wild marmosets. All marmosets were negative for <italic>M</italic>. <italic>leprae</italic> DNA, but 14 were positive for the mycobacterial rpoB gene assay. Targeted capture and sequencing of rpoB and other MTBC genes validated the presence of mycobacterial DNA in these samples and revealed that qPCR is useful for identifying mycobacterial-infected animal hosts.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Greger, M" uniqKey="Greger M">M Greger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolfe, Nd" uniqKey="Wolfe N">ND Wolfe</name></author><author><name sortKey="Dunavan, Cp" uniqKey="Dunavan C">CP Dunavan</name></author><author><name sortKey="Diamond, J" uniqKey="Diamond J">J Diamond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zakham, F" uniqKey="Zakham F">F Zakham</name></author><author><name sortKey="Aouane, O" uniqKey="Aouane O">O Aouane</name></author><author><name sortKey="Ussery, D" uniqKey="Ussery D">D Ussery</name></author><author><name sortKey="Benjouad, A" uniqKey="Benjouad A">A Benjouad</name></author><author><name sortKey="Ennaji, Mm" uniqKey="Ennaji M">MM Ennaji</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Klaus, Hd" uniqKey="Klaus H">HD Klaus</name></author><author><name sortKey="Wilbur, Ak" uniqKey="Wilbur A">AK Wilbur</name></author><author><name sortKey="Temple, Dh" uniqKey="Temple D">DH Temple</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wilbur, Ak" uniqKey="Wilbur A">AK Wilbur</name></author><author><name sortKey="Engel, Ga" uniqKey="Engel G">GA Engel</name></author><author><name sortKey="Rompis, A" uniqKey="Rompis A">A Rompis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Montali, Rj" uniqKey="Montali R">RJ Montali</name></author><author><name sortKey="Mikota, Sk" uniqKey="Mikota S">SK Mikota</name></author><author><name sortKey="Cheng, Li" uniqKey="Cheng L">LI Cheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brusasca, Pn" uniqKey="Brusasca P">PN Brusasca</name></author><author><name sortKey="Peters, Rl" uniqKey="Peters R">RL Peters</name></author><author><name sortKey="Motzel, Sl" uniqKey="Motzel S">SL Motzel</name></author><author><name sortKey="Klein, Hj" uniqKey="Klein H">HJ Klein</name></author><author><name sortKey="Gennaro, Ml" uniqKey="Gennaro M">ML Gennaro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Griffith, S" uniqKey="Griffith S">S Griffith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Truman, Rw" uniqKey="Truman R">RW Truman</name></author><author><name sortKey="Singh, P" uniqKey="Singh P">P Singh</name></author><author><name sortKey="Sharma, R" uniqKey="Sharma R">R Sharma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Donham, Kj" uniqKey="Donham K">KJ Donham</name></author><author><name sortKey="Leininger, Jr" uniqKey="Leininger J">JR Leininger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valverde, Cr" uniqKey="Valverde C">CR Valverde</name></author><author><name sortKey="Canfield, D" uniqKey="Canfield D">D Canfield</name></author><author><name sortKey="Tarara, R" uniqKey="Tarara R">R Tarara</name></author><author><name sortKey="Esteves, Mi" uniqKey="Esteves M">MI Esteves</name></author><author><name sortKey="Gormus, Bj" uniqKey="Gormus B">BJ Gormus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyers, Wm" uniqKey="Meyers W">WM Meyers</name></author><author><name sortKey="Gormus, Bj" uniqKey="Gormus B">BJ Gormus</name></author><author><name sortKey="Walsh, Gp" uniqKey="Walsh G">GP Walsh</name></author><author><name sortKey="Baskin, Gb" uniqKey="Baskin G">GB Baskin</name></author><author><name sortKey="Hubbard, Gb" uniqKey="Hubbard G">GB Hubbard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Traub, Rj" uniqKey="Traub R">RJ Traub</name></author><author><name sortKey="Monis, Pt" uniqKey="Monis P">PT Monis</name></author><author><name sortKey="Robertson, Id" uniqKey="Robertson I">ID Robertson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miller, M" uniqKey="Miller M">M Miller</name></author><author><name sortKey="Terrell, S" uniqKey="Terrell S">S Terrell</name></author><author><name sortKey="Lyashchenko, K" uniqKey="Lyashchenko K">K Lyashchenko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Truman, Rw" uniqKey="Truman R">RW Truman</name></author><author><name sortKey="Andrews, Pk" uniqKey="Andrews P">PK Andrews</name></author><author><name sortKey="Robbins, Ny" uniqKey="Robbins N">NY Robbins</name></author><author><name sortKey="Adams, Lb" uniqKey="Adams L">LB Adams</name></author><author><name sortKey="Krahenbuhl, Jl" uniqKey="Krahenbuhl J">JL Krahenbuhl</name></author><author><name sortKey="Gillis, Tp" uniqKey="Gillis T">TP Gillis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez, An" uniqKey="Martinez A">AN Martinez</name></author><author><name sortKey="Britto, Cfpc" uniqKey="Britto C">CFPC Britto</name></author><author><name sortKey="Nery, Jac" uniqKey="Nery J">JAC Nery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harkins, Km" uniqKey="Harkins K">KM Harkins</name></author><author><name sortKey="Buikstra, Je" uniqKey="Buikstra J">JE Buikstra</name></author><author><name sortKey="Campbell, T" uniqKey="Campbell T">T Campbell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thorne, N" uniqKey="Thorne N">N Thorne</name></author><author><name sortKey="Borrell, S" uniqKey="Borrell S">S Borrell</name></author><author><name sortKey="Evans, J" uniqKey="Evans J">J Evans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mendes Pontes, Ar" uniqKey="Mendes Pontes A">AR Mendes Pontes</name></author><author><name sortKey="Normande, Ic" uniqKey="Normande I">IC Normande</name></author><author><name sortKey="Fernandes, Aca" uniqKey="Fernandes A">ACA Fernandes</name></author><author><name sortKey="Rosas Ribeiro, Pf" uniqKey="Rosas Ribeiro P">PF Rosas Ribeiro</name></author><author><name sortKey="Soares, Ml" uniqKey="Soares M">ML Soares</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chalmers, Dt" uniqKey="Chalmers D">DT Chalmers</name></author><author><name sortKey="Murgatroyd, Lb" uniqKey="Murgatroyd L">LB Murgatroyd</name></author><author><name sortKey="Wadsworth, Pf" uniqKey="Wadsworth P">PF Wadsworth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Truman, Rw" uniqKey="Truman R">RW Truman</name></author><author><name sortKey="Krahenbuhl, Jl" uniqKey="Krahenbuhl J">JL Krahenbuhl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J Sambrook</name></author><author><name sortKey="Russell, Dw" uniqKey="Russell D">DW Russell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moura, Rs" uniqKey="Moura R">RS Moura</name></author><author><name sortKey="Calado, Kl" uniqKey="Calado K">KL Calado</name></author><author><name sortKey="Oliveira, Mlw" uniqKey="Oliveira M">MLW Oliveira</name></author><author><name sortKey="Buhrer Sekula, S" uniqKey="Buhrer Sekula S">S Bührer-Sékula</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duthie, Ms" uniqKey="Duthie M">MS Duthie</name></author><author><name sortKey="Goto, W" uniqKey="Goto W">W Goto</name></author><author><name sortKey="Ireton, Gc" uniqKey="Ireton G">GC Ireton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Malukiewicz, J" uniqKey="Malukiewicz J">J Malukiewicz</name></author><author><name sortKey="Boere, V" uniqKey="Boere V">V Boere</name></author><author><name sortKey="Fuzessy, Lf" uniqKey="Fuzessy L">LF Fuzessy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyer, M" uniqKey="Meyer M">M Meyer</name></author><author><name sortKey="Kircher, M" uniqKey="Kircher M">M Kircher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kircher, M" uniqKey="Kircher M">M Kircher</name></author><author><name sortKey="Sawyer, S" uniqKey="Sawyer S">S Sawyer</name></author><author><name sortKey="Meyer, M" uniqKey="Meyer M">M Meyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maricic, T" uniqKey="Maricic T">T Maricic</name></author><author><name sortKey="Whitten, M" uniqKey="Whitten M">M Whitten</name></author><author><name sortKey="P Bo, S" uniqKey="P Bo S">S Pääbo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, H" uniqKey="Li H">H Li</name></author><author><name sortKey="Durbin, R" uniqKey="Durbin R">R Durbin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huson, Dh" uniqKey="Huson D">DH Huson</name></author><author><name sortKey="Auch, Af" uniqKey="Auch A">AF Auch</name></author><author><name sortKey="Qi, J" uniqKey="Qi J">J Qi</name></author><author><name sortKey="Schuster, Sc" uniqKey="Schuster S">SC Schuster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schuenemann, Vj" uniqKey="Schuenemann V">VJ Schuenemann</name></author><author><name sortKey="Singh, P" uniqKey="Singh P">P Singh</name></author><author><name sortKey="Mendum, Ta" uniqKey="Mendum T">TA Mendum</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Patrocinio, Lg" uniqKey="Patrocinio L">LG Patrocinio</name></author><author><name sortKey="Goulart, Imb" uniqKey="Goulart I">IMB Goulart</name></author><author><name sortKey="Goulart, Lr" uniqKey="Goulart L">LR Goulart</name></author><author><name sortKey="Patrocinio, Ja" uniqKey="Patrocinio J">JA Patrocinio</name></author><author><name sortKey="Ferreira, Fr" uniqKey="Ferreira F">FR Ferreira</name></author><author><name sortKey="Fleury, Rn" uniqKey="Fleury R">RN Fleury</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Da Rosa, Fb" uniqKey="Da Rosa F">FB da Rosa</name></author><author><name sortKey="De Souza, Vc" uniqKey="De Souza V">VC de Souza</name></author><author><name sortKey="De Almeida, Tap" uniqKey="De Almeida T">TAP de Almeida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Almeida, Ec" uniqKey="De Almeida E">EC de Almeida</name></author><author><name sortKey="Martinez, An" uniqKey="Martinez A">AN Martinez</name></author><author><name sortKey="Maniero, Vc" uniqKey="Maniero V">VC Maniero</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Sharma, R" uniqKey="Sharma R">R Sharma</name></author><author><name sortKey="Lahiri, R" uniqKey="Lahiri R">R Lahiri</name></author><author><name sortKey="Scollard, Dm" uniqKey="Scollard D">DM Scollard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drummond, A" uniqKey="Drummond A">A Drummond</name></author><author><name sortKey="Nicholls, Gk" uniqKey="Nicholls G">GK Nicholls</name></author><author><name sortKey="Rodrigo, Ag" uniqKey="Rodrigo A">AG Rodrigo</name></author><author><name sortKey="Solomon, W" uniqKey="Solomon W">W Solomon</name></author><author><name sortKey="Buck, Ce" uniqKey="Buck C">CE Buck</name></author><author><name sortKey="Millard, A" uniqKey="Millard A">A Millard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ginolhac, A" uniqKey="Ginolhac A">A Ginolhac</name></author><author><name sortKey="Rasmussen, M" uniqKey="Rasmussen M">M Rasmussen</name></author><author><name sortKey="Gilbert, Mt" uniqKey="Gilbert M">MT Gilbert</name></author><author><name sortKey="Willerslev, E" uniqKey="Willerslev E">E Willerslev</name></author><author><name sortKey="Orlando, L" uniqKey="Orlando L">L Orlando</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez, An" uniqKey="Martinez A">AN Martinez</name></author><author><name sortKey="Talhari, C" uniqKey="Talhari C">C Talhari</name></author><author><name sortKey="Moraes, Mo" uniqKey="Moraes M">MO Moraes</name></author><author><name sortKey="Talhari, S" uniqKey="Talhari S">S Talhari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Beyene, D" uniqKey="Beyene D">D Beyene</name></author><author><name sortKey="Aseffa, A" uniqKey="Aseffa A">A Aseffa</name></author><author><name sortKey="Harboe, M" uniqKey="Harboe M">M Harboe</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Brésil</li><li>États-Unis</li></country><region><li>Arizona</li><li>Louisiane</li><li>Minas Gerais</li><li>Pernambuco</li><li>État de Rio de Janeiro</li></region><settlement><li>Rio de Janeiro</li></settlement></list><tree><country name="États-Unis"><region name="Arizona"><name sortKey="Housman, Genevieve" sort="Housman, Genevieve" uniqKey="Housman G" first="Genevieve" last="Housman">Genevieve Housman</name></region><name sortKey="Malukiewicz, Joanna" sort="Malukiewicz, Joanna" uniqKey="Malukiewicz J" first="Joanna" last="Malukiewicz">Joanna Malukiewicz</name><name sortKey="Stone, Anne C" sort="Stone, Anne C" uniqKey="Stone A" first="Anne C." last="Stone">Anne C. Stone</name><name sortKey="Truman, Richard" sort="Truman, Richard" uniqKey="Truman R" first="Richard" last="Truman">Richard Truman</name></country><country name="Brésil"><region name="Minas Gerais"><name sortKey="Malukiewicz, Joanna" sort="Malukiewicz, Joanna" uniqKey="Malukiewicz J" first="Joanna" last="Malukiewicz">Joanna Malukiewicz</name></region><name sortKey="Boere, Vanner" sort="Boere, Vanner" uniqKey="Boere V" first="Vanner" last="Boere">Vanner Boere</name><name sortKey="Grativol, Adriana D" sort="Grativol, Adriana D" uniqKey="Grativol A" first="Adriana D." last="Grativol">Adriana D. Grativol</name><name sortKey="Pereira, Luiz Cezar M" sort="Pereira, Luiz Cezar M" uniqKey="Pereira L" first="Luiz Cezar M." last="Pereira">Luiz Cezar M. Pereira</name><name sortKey="Ruiz Miranda, Carlos R" sort="Ruiz Miranda, Carlos R" uniqKey="Ruiz Miranda C" first="Carlos R." last="Ruiz-Miranda">Carlos R. Ruiz-Miranda</name><name sortKey="Silva, Ita De Oliveira E" sort="Silva, Ita De Oliveira E" uniqKey="Silva I" first="Ita De Oliveira E" last="Silva">Ita De Oliveira E Silva</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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